The SILCS approach to modeling protein – excipient interactions to predict biologics formulations

The SILCS-Biologics toolset utilizes the SILCS platform technology in a novel way, to direct and inform in silico biologics formulations through mapping of protein-protein interactions and comprehensive excipient modeling. The technology is applicable to all classes of proteins, including monoclonal antibodies (mABs).

A computational approach to expediting the biologics formulation process

Biologics are formulated to maintain their activity during long-term storage and subsequent administration. Maintaining their 3D conformations and preventing protein aggregation is an important challenge in their development. Experiments to identify the optimal combinations of excipients, buffers, and surfactants are low-throughput and require high concentrations of protein – which is limiting and costly. Failure to determine the optimal formulation results in decreased product performance, impacting patient treatment and, in some cases, requiring the costly reengineering of the therapeutic protein.

SILCSBIO INNOVATION

SilcsBio has developed a novel in silico formulation design technology to inform the selection of desired excipient/buffer combinations. Using the established SILCS platform, SilcsBio’s technology 3D maps the protein of interest (FragMap) and its functional group affinity pattern, setting the foundation for evaluation of the full range of protein-protein interactions and the full distribution of buffers and excipients on the protein. This data may be used to rationally enhance stability, limit viscosity, and prevent aggregation.

SILCS-BIOLOGICS GUI

The SILCS-GUI provides a point-and-click approach to protein system preparation and SILCS simulation job submissions for FragMap generation. After the simulations are complete, the GUI allows for ready access to the full capabilities of the technology.

SILCS-BIOLOGICS CAPABILITIES

SILCS-Biologics utilizes the FragMaps to perform mapping of protein-protein interactions (PPI) and the distribution and affinity of excipients and buffers on the entire protein surface. That information is then combined, yielding an information-rich dataset that may be used to direct biologics formulation, including interpretation of experimental data and predictions of excipients that could modulate desirable properties of the biologic formulation. This technology has been applied to a range of protein biologics, including monoclonal antibodies.

BENEFITS

The SILCS technology enables screening of hundreds of excipients and buffers, as well as protein-protein interaction (PPI), within a day, minimizing the need for experimental screening.

Rational Selection

Excipient/buffer combinations in silico guided by SilcsBio’s approach, lowering costs and truncating timelines for developing novel biologics.

Stability

Improved formulations to better maintain the stability of biologics during storage and delivery.

ACCELERATE YOUR RESULTS WITH SILCSBIO

Connect with us today to learn more about how we can help your team work faster and more efficiently.

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